Human Cloning Essay Example | Topics and Well Written Essays - 750 words

Human Cloning - Essay Example 7). Human cloning is a controversial that elicits diverse reactions from all quarters and almost every member of the human race. The issue of cloning has caused much debate since the firs cloning of a live sheep in Scotland in 1997m due to ethics and authority to participate in the events leading to the creation of a new human being from the originally born one. This topic is crucial to the existence of human beings as it may be used to either improve their lives or degrade them. Personal Opinion In my opinion, human cloning is a gift that should be embraced by all of humanity because it has multiple benefits if made use of in appropriate ways. Cloning should be allowed among humans because it can be used to cure or improve certain conditions such as infertility. In reference to this, infertile couples can have children of their own. In addition, cloning, if allowed can be used to repair damaged tissues and organs in the human body. This is through growing new organs, which is called therapeutic cloning; that can then be used for the maintenance of the human body. This is especially so for conditions that requires transplants such as leukemia, liver and kidney failures (Human Genome Project Information, 2009, Para. 15). Cloning can be used to generate new bone marrow that can then be transplanted into the patient and, therefore, healing them of their condition. In addition, it lessens the risk and burden that loved ones have to bear in order to save their lives. This is in the form of surgery that they have to go trough so as to donate their organs for use by others. Not only can human cloning be used in the above named processes, but also in plastic and reconstructive surgeries. Cloning allows for production of body tissues that are compatible with the host, which in turn lessens the risk of tissue rejection (Advancing Science, Serving Society, 2007, Para. 5). For this reason, cloning can be used to treat conditions such as amputated limbs as they may easily b e regenerated. The additional conditions that can benefit from human cloning include defective genes and genetic diseases. This would be beneficial, especially to parents that have genetic disorders. For parents considering conception, they may see a specialist to have the genes eradicated and thus ensure that they do not pass the problems on to their offspring. People Opinions The majority of those who think that human cloning should be allowed share common belief with me that it can be used for the good of human kind, and especially so in regard to health. One opinion that is outstanding is that, due to the preservation of health and even extension of life, scientists could continue to live. As a result, the world would benefit from their great discoveries and inventions. All this would be the result of recreating their minds in a different time and body. For example, if this were to happen to the Wright brothers, they would help greatly in developing space exploration and the avi ation industry at large. Furthermore, others claim that for the purpose of reproducing offspring in cases of couples who cannot conceive, cloning should be legal, but only if the child will not be subjected to tests for his or her entire life. Therefore, it should be perfected before it is used for such events. In spite of all their well-argued reasons for supporting human cloning, some reasons for their support were utterly misconceived and based on fallacies of over imagination. One such thought was that cloning should be allowed in order to counter population problems. The basis of this argument was that; height and weight be regulated in order to fit people into certain special buildings. This beat the entire purpose of cloning as only clones were to be fitted into these â€Å"

The Muslim World Essay Example for Free

The Muslim World Essay World is divided into different divisions depending upon the economic conditions, race, culture and most importantly religion. The division of world is more evident after the September 11 attacks into Muslim world and anti-Muslim world. The political ideology and attitudes were now deeply rooted among the individuals also. â€Å"The anti-Muslim attitudes of West united the Muslim world â€Å"is a blanket statement and have strong relevance to the present day. Western attitude: The conflict between in not a new one, it is continuing from 14 centuries some of the conflicts of the history are Ottoman Turkish empire expansion, quasi-colonial regimes by Britain and France, Britain occupation of Iran etc. The first and most important issue is the stand of United States in the ‘Israeli-Palestinian’ Conflict. The need to explain the policies of it on the issue were undermined and unheard. The role of the Western media which is publishing derogatory statements is also a cause of concern. US media used the terms such as Muslim fundamentalist, Shia extremist, Wahhabi zealots, Islamic Jihad, Islamic suicide bomber, Arab killer etc (Ali Khalid, 2008). The Muslim world is victimized more than the situation. Many allegations made by the West on different Muslim countries without any proofs. West failed in providing the evidence of the allegations after the war against the Iraq and its shift in the reasons for war, Muslim prisoners were tortured even after the wars, prolonged occupation etc are also serving as anti-American resentment (Duiker Spielvogel, 2009). Strong factors that united Muslim World: The anti-Muslim attitude united the Muslim world and started to have a common perception as a whole on West. The main reasons for this is unfavorable images where the Muslims are unfairly portrayed in media, Western Military presence in middle east making them U. S long term military base brining insecurity in the region, The containment policy toward Iraq with economic and military sanctions on it, the strong U. S policy towards the Arab-Israeli conflict, Support of the West to unpopular regimes etc. All these factors are allowing the Muslim world to rethink on their status ( Prados, 2001). From the Western perspective the main reasons for the anti-American resentment in the Muslim world is due to education, media and Anti-Americanism (Gentzkow, Shapiro, 2004). West counters: Even West is taking some of the measures to overcome this anti-American negative approach such as implementing the trade and aid programs to decrease economic inequalities, to change the unfavorable image, seeking support on U. S policy on Iraq, planning to developing new strategy on Arab-Israeli peace negotiations, influence positive open political systems etc. Conclusion: U. S is successful in brining gap between the Muslim world to an extent where it divided it into US allies, US enemies and Neutral countries basing on its relations. As a whole West is using all its strategies to change its image and forcing the Muslim world to react to against terrorism, the Muslim world persecution is different. But carefully watching Muslim world is aware of the West motives and started to have a common agenda and this will be fruitful in coming days. The statement â€Å"The anti-Muslim attitudes of West united the Muslim world† is justified by looking at the anti-Muslim factors causing the unification of Muslim world. Even though the West and U. S in particular are taking the measure to have a better image in the Muslim World, the Muslim community is expecting more and concrete steps at ground level rather than confining to the media statements. Annotated Bibliography: Ali, S Khalid (2008). Mass Media and Muslim World: Portrayal of Muslim by â€Å"News Week† and â€Å"Time† (1991-2001). European Journal of Scientific Research, 21, 554-580. This is a research article which examines the representation and coverage of 12 Muslim countries. This investigation was done by the Time Magazine and the New Week during 1991-2001. This article has presented content analysis of 218 articles which belongs to 12 Muslim countries. Duiker, W. J Spielvogel, J. J. (2009). World History, Volume 2. 6th ed. MA: Cengage Learning. This book provides an overview of world history which discovers experiences and challenges to recognize global patterns. This book covers each and every aspect like political, economical, religious, social, cultural, intellectual and military history. It takes a global approach by comparing cultures throughout world history. This book helped to know the Muslim World perception on the U. S and also how U. S intervened in the internal aspects of Middle East. Prados, A. B. (2001). Middle East: Attitudes toward the United States. Congressional Research Service. Retrieved from: http://fpc. state. gov/documents/organization/7858. pdf This report discusses the attitude of Muslims and Arabs of Middle East against United Nations. It explained the factors which have created hatred towards the United States and the methods to deal with this position. Gentzkow, M. A. Shapiro, J. M. (2004). Media, Education and Anti-Americanism In the Muslim World. Journal of Economic Perspectives, 18, 117–133. This paper is based on the media, education and anti-Americanism in the Muslim world by taking a survey of 10,000 people from nine Muslim countries. It examines how media use and education relate to the positivity towards United States and their belief.

Nature vs Nurture in Mary Shelley’s Frankenstein Essay -- Mary Shelley

Philosophers and scientists alike have debated for centuries whether a person’s character is the result of nature or nurture. In the writings of Thomas Hobbes, it is expressed that humans are endowed with character from birth, and that they are innately evil in nature. John Locke’s response to this theory is that everyone is born with a tabula rasa, or blank slate, and then develops character after a series of formative experiences. The idea that true character is the result of experiences and societal interaction is a theme deeply explored throughout Mary Shelley’s Frankenstein. Through different interactions with the monster, Shelley attempts to express that it is because of Victor’s failings as a parent and creator, because of the monster’s isolation, and because of society’s reaction to the monster that the monster has become evil. The monster’s character is a direct result of how he was nurtured, based on his experiences and circ umstances, rather than his being innately evil from â€Å"birth.† One of the most influential contributions in the formation of the monster’s character is Victor’s failure as a creator and a father. As a creator, Victor has the responsibility of providing for his creation, just as God provided for Adam and Eve. At the same time, Victor also falls under the role of a father, and should therefore seek to strengthen the familial bond between the two of them. However, Victor fails in both of these endeavors, because he cannot accept the monster in his deformity. â€Å"Frankenstein’s sole regret†¦ is that he did not create an aesthetically pleasing being† (Bond). Victor, due to his skewed vision of humanity, believes outer beauty to be a reflection of inner character, and that because of the monster’s hideous appe... ...tation.† The English Review Sept. 2009: 18+. Literature Resource Center. Web. 9 Jan. 2015. Lehman, Steven. â€Å"The Motherless Child in Science Fiction: Frankenstein and Moreau.† Science Fiction Studies 19:1 (Mar. 1992): 49-57. Rpt. In Children’s’ Literature Review. Ed. Tom Burns. Vol. 133. Gale, 2008. Literature Resource Center. Web. 9 Jan. 2015. Marcus, Steven. "Frankenstein: myths of scientific and medical knowledge and stories of human relations." The Southern Review 38.1 (2002): 188+. Literature Resource Center. Web. 9 Jan. 2015. Seabury, Marcia Bundy. "The Monsters We Create: Woman on the Edge of Time and Frankenstein." Critique 42.2 (Winter 2001): 131-143. Rpt. in Children's Literature Review. Ed. Tom Burns. Vol. 133. Detroit: Gale, 2008. Literature Resource Center. Web. 9 Jan. 2015. Shelley, Mary. Frankenstein. New York: Barnes & Noble, 2003. Print.

Affect of Women in Advertisement Essay

Abstract Due to advertisement clutter in the 21st century customers are exposed to a variety of advertising appeals that aims to influence their attitudes towards a wide range of products and services. Many companies worldwide are using women as an object to attract and influence consumers’ attitudes towa rds the products they sell. Women appear in huge number of advertisement as a sexual object or as attractive decorative model standing nearby a product, even when the sexual image has little relevance to the advertised product. This article looks at Jordan ian male consumers’ attitude towards using women in advertisement. Results reveals four factors tend to influence consumer attitude towards using women in advertisement. Culture, control, and emotion were found to have a significant influence on consumer attitude towards using women in advertisement, while there was no significant influence for women appearance on consumer attitude towards using women in advertisement. In general Jordanian male consumers’ were opposed to exposing, exploiting and objectifying the woman and her body in advertisement. Introduction Research on advertisement featuring sex role focused on how content and imagery affect cognitive responses and attitude toward the advertisement, which in turn affect attitude toward the brand and purchase intention (Jaffe, 1994; Leigh and Whitney, 1987). Attitude toward advertising can be defined in general as learned tendencies to respond in a consistently favorable or unfavorable manner to advertising in general (Lutz, 1985; Mackenzie and Lutz, 1989). The link between attitudes, intentions and behavior has been explained primarily by Ajzen (1985, 1988), Ajzen and Fishbein (1980). This theory is based on the assumption that human beings usually behave in a sensible manners where they will take into account information available to them and consider the consequences of their actions. Thus people are expected to act in accordance with their intentions. The personal factor is the attitude toward the behavior, which is the individual‟s positive or negat ive evaluation of performing the behavior of interest. Whereas the social determinant of intention is the person‟s perception of social pressure to perform the behavior under consideration. People generally intend to perform a behavior when they evaluate it positively and belief that important others think they should perform it. Advertisements perceived as contentious will not be effective in capturing the attention of people or changing their attitudes towards the advertised products (Michell and Al-Mossawi, 1995). Based on that, it‟s very essential to choose the appropriate advertisement strategies employed in advertising which range from informational to emotional. Among those designed to stir emotions or rouse particular feelings, we can find fear, humor, warmth, novelty, contrast, animation, music, and sexual arousal. According to Reichert et. al. (2001) sex appeal advertising invokes any message, which, whether as brand information in advertising contexts or as persuasive appeals in marketing contexts, is associated with sexual information. It has long been an accepted belief that this form of advertising is very effective at attention-grabbing, considered by some commentators as a powerful step in reaching one‟s target market, especially in the current clutter of 21st century marketing and communications (Reichert & Lambiase, 2003). Sexual economics theory (SET) is a stimulating theory about sexuality that combines the idea of gender differences in sexual attitudes with social exchange theory, which S precher, (1989) conceptualize as two or more parties that each give up something with the aim of getting back something of greater value. Much research suggests that sex as an end in itself is less valued by women than men (Baummeister et al., 2001). Ther efore, sexual economics theory posits that women possess substantially greater negotiating power than do men in the context of a sexual exchange. Based on this theory, the current study will focus on men and the way women are used to persuade them in adver tisement to influence their attitudes. Using women in advertisement as a sexual appeal has transferred women to a marketing instrument by many firms. Women are used as a material mostly in advertisements (Bayraktar, 2011). In many advertisements, the ideal woman is an object that exists to satisfy men‟s sexual desires. Many researchers have shown that women in advertisements are portrayed as sex objects (Baker, 2005). Advertisers feature provocative images of sexually attractive women in ads (Reichert, 2 002). Sexual appeals in advertisements consist of a variety of elements models. Courtney and Whipple (1983) describes sexuality in advertisements as sexuality in the form of nudity, sexual imagery, innuendo, and double entendre used as an ad object for dif ferent kinds of products. Reichert (2002) reveals that common forms of sexuality in ads includes nudity (dress), physical attractiveness, seductive behavior and interaction, innuendo, and other factors such as setting, context and camera effects. While Lambiase and Reichert (2003) propose that there are five types of sexual content in ads: Nudity, sexual behavior, physical attractiveness, sexual referents, and sexual embeds. Ramirez and Reichert (2000) propose that viewers consider physical characteristics the most sexual in ads and the sub-categories of these characteristics are clothing, attractiveness and body. Table 1 shows the categories of women appearance in advertisements and their meanings in USA. It‟s noteworthy to say that women misrepresentation in advertisement is a world wide phenomenon. In India, for example, Bag and Roy (2010) bring up various reasons behind unaccept ed representation of women in advertisement, such as poverty where still a number of women, particularly young girls with intolerable economic misery compelled to represent themselves in different forms of uncultured version for survival. To the contrary some rich women’s do it as a hobby, just to get mental or psychological satisfaction through modeling, front page sensational attraction in different popular magazines. Globalization is another factor responsible for misrepresentation of women in advertisement. Being attracted to western culture, teenager girls have been adopting western style in their garments and manners and hence, they have been losing their own culture and tradition, social values and moral character. Consumerism, which tries hard to inc rease the consumption of people, is another cause of such unaccepted representation. Much consumption means much economic profit which a group of people highly want. Profit maximization has escalated the introduction of women in a number of advertisements of different companies in television, magazine, T.V., Cinema, Newspaper, Cartoons, painting and even in the Internet to attract customers from different age groups. The different types of women representation in advertisement are presented below. Representation through Television- There is a lot of representation of women in advertisement through T.V. which affect common people and children adversely. It is obvious that visual indecent representation of them has been perverting psychologically all youth. Representation through Newspaper- Newspaper is another source of representation in indecent manner. Readers from almost all age group are intensely influenced while observing and reading those undesirable advertisements. Representation through Magazine- Similar examples will be cited in several advertisements in several reputed magazines where the honor of women have not only been despoiled simultaneously people are being psychologically endangered. But interestingly, by virtue of human instinct, neither presenter nor consumer is realizing its adverse impact. Other forms of Representation- There are other forms of uncultured representation which we can find rarely but have both internal and external effects on common people. Despite fewer in number, such representations are noticed in different sorts of paintings, hoarding hanging or fixed in cities and towns, and even displayed in Internet. Painting of undesirable quality is often displayed in cinema hall for greater publicity. Similarly, a lot of painters draw naked or half naked figures, which may have inner good meaning, but it is a matter of question how many people are finding that inner good meaning and how many are taking those adversely. Research Problem and Significance Very few studies tries to explain the influence of using women in advertisement based on cultural factors. Researchers have either concentrated on or compared dissimilar cultures, such as the U.S and Japan or they have focused on similarities or differences on similar cultur es, such as Great Britain and Australia (Al-Olayan and Karande, 2000). Many researches explain ads by using Hofstede‟s cultural dimensions or Hall‟s high and low typology and other country specific variables. Culture dominates communications, such as langu age, traditions, beliefs and music (Al-Olayan, & Karande, 2000). Therefore, the main motivation behind this article is to identify the influence of using women in advertisement on male consumers‟ attitude in Jordan. This study is conducted in a Muslim country where Shariah (Islamic Law) prohibit the use of sexual appearance or body in a way to attract or convince consumers with company products, brands or services. This study is significant for the reason that there is no any study has been done in this field regarding this issue. Another reason for doing this study is to increase our understanding of the Arab societies, and in this case Jordan. Research Objectives The objectives of this research are: 1) To identify the factors that may affect male Jordanian consumers‟ attitude towards using women in advertisement 2) To examine consumers overall attitude towards using sexy women in advertisement Theoretical Background Culture and Advertisement Marketers need to observe the cultural traits, environments, norms, beliefs, and values of a particular country or region to obtain an understanding of whether their future behaviors will be perceived as ethical (Haque et al., 2010). For example, companies advertising their products or services in different countries should be aware of a country‟s existing norms concerning role portrayals and the way these organizations affect these norms (Gilly, 1988). Clow and Baack (2009) maintained that culture, religion and value system determine the levels of nudity, sexual references, and gender specific issues that are permitted in a country. Several differences have also been recognized across countries for men and women appear in different settings and for different products. Women are more likely to be portrayed in domestic environment as sex objects, while men are more likely to be portrayed being occupied. In Sweden, for example advertisers show men and women in greater variety of nonworking roles than in the U.S.A and Germany and women in Asian countries are less likely to be shown as sex objects (Das, 2000). Since in many Middle Eastern countries, sex and gender issues are taboo subjects, sexual appeals are not used in advertising and even sexually related products are difficult to advertise (Bryant, 1998). Muslim nations tend to reject any kind of nudity and any reference to sexuality and other gender -related issues. Any hint of sexuality or display of the female body is strictly forbidden. In Saudi Arabia and Malaysia, women must be shown in family settings. They cannot be depicted as being carefree or desirable to the opposite sex. Marlin (2008) conduct a study on how culture affects advertisement, he used an advertisement showing a sexy picture for Paris Hilton, the famous model wearing a bikini and asked respondents to writ e their reactions. About 27% had positive reactions, 45% had negative reactions and 28% were indifferent. For this advertisement some respondents were confused whether it is an advertisement for a burger or for a car. The results show that different cultures imply different reactions, some of the comments the respondents wrote were: †¢ Unrealistic, bizarre (German respondent) †¢ It is not about the product, they are selling a lifestyle (Iranian respondent) †¢ Nice, interesting (French respondent) †¢ Too much naked skin (1 Ethiopian and 1 Swedish respondent) †¢ I am discussed, angry and feel fat (Swedish respondent) †¢ Too naked, too unrealistic (Moroccan respondent) †¢ Sexistic (Swedish respondent) †¢ Inappropriate, bad taste (French respondent) †¢ It is totally unnecessary for the object of advertising (Swedish respondent) Muslim countries are not the only ones with restrictive advertising for sex appeals. Many Christian countries such as Ireland, Spain, South Africa, Mexico and the Philippines have similar standards. 184 While in Canada, France, and Sweden, sexism should be avoided in any advertising directed toward children (Boddewyn, 1991). Based on that, understanding how religious beliefs influence the attitude towards the advertising is of great importance to international advertisers and advertising agency managers in their efforts to improve advertising effectiveness without offending or alienating their target audience. It‟s important to comprehend that Islamic social philosophy is based on the belief that all spheres of life: spiritual, social, political, and economic form an indivisible unity that must be thoroughly imbued with Islamic values. Advertisement and Women in the Muslim world The aim of this section is to give an in-depth understanding on the nature and position of women and advertisement in the Muslim world to help and benefit marketers and the advertising producers in the Muslim world to gain a profound understanding of Muslim values. As a starting point, it‟s important to note that the Holy Quran does not prohibit advertising (Al-Makaty et al., 1996). Some advertisements use religious terminology to reassure consumers of the Islamic integrity of products and services. For example, in a television advertisement, a Saudi Investment ba nk in Egypt had used religious terms to show that it had no unlawful transactions and that all of its financial products Shariah compliant. The use of selected Quranic injunctions and words can enhance the mood of the advertising communication to make it more appealing to Muslim consumers. Luqmani et al. (1989) provide an example of a manufactured water pump that uses a verse from the Quran in advertising. Although it is obviously welcome to keep in mind that the Islamic regulations related to business and marketing, appropriate implementation of precise Islamic terms is crucial. Contrary to this, a business may face unexpected troubles in gaining markets in Muslim populous areas if some how its advertisements create feelings that are inconsistent with religious sensitivities. In such a context, the most potential and contemporary issues in Islamic markets should be carefully introduced, developed, and offered through appropriate advertising and communication messages which meet the commitment of ethics as gu ided in the Islamic Shariah. For example, the Islamic system encourages moderation in living, while prodigality and waste are denounced. Costly promotional efforts and massive commercial advertisements by businesses are considered unnecessary. Producers of advertisements must make sure that their advertising campaign does not overstep any social or legal norms. Little attention has been paid on the exposure of advertisement in the Arab societies. Except from investigations in Saudi Arabian ads, there is only one study that has examined the cross-cultural differences in advertising content in other countries in the Middle East and only one study of the portrayal of Egyptian women in television commercials. Luqmani et al., (1987) study focused only on the influence of Islam on advertising content and regulation in Saudi Arabia while Michell and Al-Mossawi, (1999) study focused on message contentiousness among Gulf Co-operative Council countries, and showed that religious Muslims scored lower in terms of recall and were unfavorable towards contentious advertisements relative to lenient Muslims. The findings suggest that there is a difference in perceived controversial elements in advertisements between a devout and a lenient Muslim. These findings also highlight the importance of matching creative execution and message content to a society‟s socio-cultural environment. Michell and Al-Mossawi claim an offensive advertisement will not be effective in capturing an audience‟s attention or changing his/her attitudes. Both of these studies revealed the importance of understanding the Islamic religion in relation to effective advertising. In particular, Luqmani et al., assert that unconventional advertisements must obtain prior approval from religious authorities. While in Malaysia, Unilever‟s used an ad for Pond‟s skin lightening moisturizer showing a Malay college student using the product for a fairer complexion to attract a boy‟s attention. The ad is deemed offensive to the Muslim ethnic culture (Haque et al., 2010). In addition to the above mentioned facts, the women‟s status is debated among traditional Islamists, reformist Islamists and feminists, secular or Islamic. The traditional Islamists are clear about the male-female differences and insist on the distinct sex roles. They view women as emotional, sentimental and weak humans whose duty is in the household. The traditional Islamists take every verse in the Qur‟an as a source of protection of women and see women as minors that need to be protected by superior men. In return the woman will nurture the family and try to please her father, brother or son, or whoever may be the head of the household. The woman has a responsibility to maintain the honor, reputation and status of the family. Traditionally, a man‟s hon or depends on the extent on the virginity of his sisters, wife and daughters, and widows divorcees‟ sexual continent. 185 The Islamic reformists believe that the inferiority is only in relation to the woman‟s physical strength and weaker autonomy, thus she is capable of participating in all spheres or public and social life. Modern and liberal reformists are trying to accommodate the Islamic rules with the realities of modern times. The feminist view is a secular movement whose aim is to achieve equality for women. The roots of this movement consider the woman as totally subordinated to the man and that the veil is a symbol of this. Advertising in Jordan Jordan‟s media sector has seen significant privatization and liberalization efforts in recent years. Based on official rack rates, research firm Ipsos estimated that the advertisement sector spent some $303m towards publicity in Jordan‟s media, 80% of which was spent on newspapers, followed by TV, radio and magazines. In recent years, Jordan has also seen a spectacular rise in the number of blogs, websites and news portals as sources of news information. The increasing diversification of Jordan‟s media boosted advertising revenues and private initiatives. Recording growth of 30%, 2007 turned out to be yet another outstanding year for Jordan‟s advertising industry. Following nearly a decade of double-digit growth, the Jordanian advertising had some catching up to do with the rest of the region in terms of average expenditure per capita. Since 2000 total ad spend increased from $77m to $303m in 2008, an increase of 260%. The Jordanian telecoms sector was the biggest ad spender in 2008, accounting for around 20% of the market, followed by banking and finance sector (12%), services industry (11%), real estate (8%) and the automotive sector (5%). Research Design In order to measure Jordanian male consumers‟ attitude towards using women in advertisement , male respondents between 18-55 years were selected. The survey instrument included a list of 22 statements from which respondents were asked to indicate their level of personal acceptance on a five-point scale, where 1 means â€Å"Strongly Disagree† and 5 means â€Å"Strongly Agree†. Statements from 1 -17 was designed to measure factors affecting consumers attitude towards using women in advertisement. Most of the statements were adapted from earlier studies, for example (Al Makaty et al. 1996; Nordà ©n 2006; and Baker, C.N. 2005). While statement 18-22 were designed to measure consumers overall attitude towards advertisements. The statements were adapted from earlier studies as well such as (Usman, 2010; and Ashill, 2005). Respondents belonging to different demographic groups in terms of education level, marital status and profession were considered in the study. The sampling frame for the study was male consumers living in west of Amman. For respondents‟ selection, simple random sampling procedure was adopted. Questionnaires were filled by the respondents. The total number of respondents was 450. The responses received were 353 (80.5 per cent). Cronbach alpha reliability analysis was conducted for the items included in the study. The internal consistency – Cronbach alpha value for reliability of the questionnaire was found to be 0.74. All items were well above the 0.70, which was the commonly accepted threshold (Nunnally 1978). Respondent Profile Respondents from different parts of west Amman were asked to fill in the questionnaire. Table 2 depicts a summation of the study‟s respondents‟ demographic information. As per research objectives all the respondents are male consumers. Of the 353 respondents, 79.0 per cent were less than 25 years of age. A larger number of respondents were unmarried (74.4 per cent) the maximum respondents fell into this age group, since youth is the target market and they are the ones who pay maximum attention to the advertisements. Out of the remaining sample, only 2.3 per cent were above 55 years of age. The remaining 29.5 per cent belonged to the age group of 26–55 years of age. The larger number of r espondents was unmarried (74.4 per cent), and this could be attributed to the young age of the respondent. In addition most of them 78.5 percent were with undergraduate education. The maximum respondents in the category „profession‟ belonged to the student group with a major share of 68.2 per cent and the remaining 31.8 per cent included businessmen, executives and others. The overall composition of respondents included in the study was young, unmarried and students.

Personal Response on Sexual Identity

I am guided by the pursuit of pleasure because I prefer to do what feels right rather than what is best. I answer to my gut feeling or listen to my heart if you will. Was raised to do what Is best for me and not for anyone else, and have also taught myself never regret anything that I do but learn and grow from my mistakes. I also relate to the value rationalism, which may seem like I contradict myself but sometimes I do stop and think about the consequences before I act instead of living in the moment. In my past, I have made some terrible mistakes and has made me want to be more cautious about certain situations I come across.I believe that I will always relate to these values. Critical Thinking and Sexual Decision Making People may not know that they use critical thinking every time they make a sexual decision, but I know how Important It Is to think a sexual decision through before making a decision. I would consider myself a skeptic even after this class. Being a skeptic means t hat I have to question anything related to sexual activities. I find it important to think about the consequences before making a decision because I can avoid making mistakes.A time that I had to use critical thinking was when I was deciding whether to lose my virginity or not. That decision was not something that I could choose right away but would take me a while to think about the consequences. It was my decision ultimately to make, and no one could help me decide. Critical thinking is a must anytime you are about to make a life-altering decision. Gender Identity Gender identity is the belief in which you truly are. Before we are born we are given a sex assignment, based on the internal and external organs we have in the womb.As e grow up, we learn whom we are and determine which gender we agree with. Most of the time we identify with the gender we were assigned with when In the womb or after birth. There are times that Individuals choose the opposite gender than the one they wer e assigned. Factors that help determine gender identity is genetic factors, social factors and environmental factors. Between man and woman, 23 chromosomes are combined to make up the genetic code. The egg carriers the X sex chromosome and the sperm can carry either the X or Y sex chromosome. This is the sex assignment and usually the gender most people identify as.Social factors help they raised you as a boy, you would most likely identify yourself as a boy. Also, your peers can have an effect on how you determine your gender identity. If one wants to fit in, he or she may try to identify as the gender that their peers are. Environmental factors can help determine your gender identity by naturally shaping you into whom you believe you are. People go through many situations that can have an effect on your gender identify. The factors that have affected my gender identity are genetic factors, environmental factors and social factors.At birth, it was determined that I m a girl because of my internal and external organs I have. Social and environmental factors determined my gender identity because my parents raised me based on my sex assignment. My parents and our society taught me that there was only one way. Which technically meant that I had no say in my gender identity since I was born as a girl I was to remain a girl. The factor that most helps me to determine my gender identity was the social factor of my parents raising me as I was meant to be. Masculinity and Femininity On the continuum of masculinity-femininity, I fall close to the middle but closer to Minnie.I like to get dressed up and be â€Å"girl' with makeup and purses, but I also like to hang with the guys and watch football and drink a beer. I would not say I am masculine in any way, but I do have masculine tendencies because I'm not afraid to get dirty. I grew up with both parents so I would say they balanced my feminine and masculine qualities. They taught me the important parts of being femin ine and masculinity. My mother taught me to act like a lady, and my father taught me how to change a tire. Attraction There are three components of attractiveness; they are attitude, physical attraction ND reciprocity.The first thing someone notices about another person is his or her physical appearance. What makes a person want to further the relationship is his or her attitude. If they find the attitude to be attractive, chances are they will try to further the relationship. Reciprocity is when both parties want the same thing and will work together to make their relationship a lasting one. The factor that is most important to me would be an attitude because while physical attraction is the first thing I notice, attitude is the component that makes me want to act on my feelings.If someone does not have a good attitude or a similar attitude as mine, I will not find him or her attractive. Styles of Love The styles of love that are constant in my life right now are romantic love, pos sessive excited love and friendship. Romantic love has always been a constant in my life because I am a lover. When I fall in love, I fall hard. Romantic love is probably the truest love today. Possessive excited love is a style of love that I find in my life today. Although my boyfriend and I are not possessive, we have a lot of excitement in our relationship.The excitement to see each other after a long day of work or the excitement to have a date night keeps our relationship strong. Before my boyfriend and I decided to be in a relationship, we were really great friends. We bonded instantly and got along very well. When I started to feel like being friends was not enough for me, I had to let him know. Historical and Scientific Perspectives on Homosexuality Homosexuality is when an individual is attracted sexually or romantically to people of in our society we have people who protest against gays having equal rights such as arraign.Of course, our society has come a long way with he terosexuals fighting along side the homosexuals for the their rights. Historical and scientific perspectives have shaped the way I perceive my sexual orientation and myself. Historically, homosexual practices were either done in secret or known about but shunned. Homosexuals were looked down on and seen as abominations. In the past individuals who were accused of homosexuality were convicted by the â€Å"Office of the Night†, which was a group of Christians who felt negatively on homosexuals.In today's society, homosexuals do not have to hide that they are gay because they can not be convicted for whom they are. Unfortunately, there are still individuals out there that do not agree with homosexuality. There is still the potential of gay individuals being harassed by those individuals. Hate crimes are still common in our society. I am not gay, but I do have gay friends and family, and I do not look at them any differently; instead I stand beside them and protect them from any hate that comes their way. History has only taught me that we need to fight for the rights and that I need to be true to myself.Scientific perspectives have researched the possibility of genetics and mental health being the cause of homosexuality. They have studied family trees to determine if homosexuality can be passed down through generations. They only found that the X chromosome could influence sexual orientation. The researchers have also studied the brain and the possibility that homosexuality can be caused by a mental illness of some sort. Scientific perspectives have not reflected on my sexual orientation. I am straight because I choose to be.I believe that anyone should be able to love whoever they want without being Judged for it. Abortion When I was younger I always thought abortion was wrong because it was taking a life of a baby. As I grew up and learned more, I realized I was making my decision on abortion based off of others ideas of abortion. I became aware of abort ion when a friend of mine in high school had an abortion after having sex once. I understood why she would want an abortion because of being in high school and she had her whole life ahead of her. Her family was very strict, and she was worried about her parents disowning her.

Free Essays on I Never Learned Their Names

The Unpublished Ellison In the short story â€Å"I Never Learned Their Names† has many layers, and multiple meanings to people in different situations. This Ellison story relates to his â€Å"childhood, and his early examples of his lifelong fascination with American identity.† As I read the story I was confused about the exact underlying meanings. This is because I hadn’t looked into the story as it related to his early life. However when read again I began to see three main underlying meanings. The Underlying meanings are the personal issues that Ellison dealt with in his life, the journeys that Ellison took and cultures society in which he was living in. The first underlying meaning that I came across in the story â€Å"I Never Learned Their Names† was the personal issues that Ellison dealt with in his life. The main two issues that I saw Ellison dealing with was that he was a black man when racism was a major part of the life style, and also his problem that he had with money. Ralph being a black person was the first personal issue that I noticed. In the second paragraph of this story he writes that his buddy â€Å"got quite a kick out of having a Negro for a buddy. Why should it matter if somebody has a black friend, white friend, or a cream colored friend? A friend is a friend and you shouldn’t get a kick out of the color of there skin, but the personality in which they have. However, when Ellison was traveling it didn’t matter and Morrie had a lot of courage to be friends with Ellison. The second personal issue that I felt that Ellison had to deal with was his lack of money, which also derives from his childhood. His mother passed away and he and his brother were left with little money. In the story he has been traveling around trying to find work, so that he could get money to get an education. He had been many places and was on his way to Alabama hoping to find work. He and his friend Morrie got food from people on the... Free Essays on I Never Learned Their Names Free Essays on I Never Learned Their Names The Unpublished Ellison In the short story â€Å"I Never Learned Their Names† has many layers, and multiple meanings to people in different situations. This Ellison story relates to his â€Å"childhood, and his early examples of his lifelong fascination with American identity.† As I read the story I was confused about the exact underlying meanings. This is because I hadn’t looked into the story as it related to his early life. However when read again I began to see three main underlying meanings. The Underlying meanings are the personal issues that Ellison dealt with in his life, the journeys that Ellison took and cultures society in which he was living in. The first underlying meaning that I came across in the story â€Å"I Never Learned Their Names† was the personal issues that Ellison dealt with in his life. The main two issues that I saw Ellison dealing with was that he was a black man when racism was a major part of the life style, and also his problem that he had with money. Ralph being a black person was the first personal issue that I noticed. In the second paragraph of this story he writes that his buddy â€Å"got quite a kick out of having a Negro for a buddy. Why should it matter if somebody has a black friend, white friend, or a cream colored friend? A friend is a friend and you shouldn’t get a kick out of the color of there skin, but the personality in which they have. However, when Ellison was traveling it didn’t matter and Morrie had a lot of courage to be friends with Ellison. The second personal issue that I felt that Ellison had to deal with was his lack of money, which also derives from his childhood. His mother passed away and he and his brother were left with little money. In the story he has been traveling around trying to find work, so that he could get money to get an education. He had been many places and was on his way to Alabama hoping to find work. He and his friend Morrie got food from people on the...

How Eli Whitney Invented the Cottin Gin

How Eli Whitney Invented the Cottin Gin Eli Whitney was the inventor of the cotton gin and a pioneer in the mass production of cotton. Whitney was born in Westboro, Massachusetts on December 8, 1765, and died on January 8, 1825. He graduated from Yale College in 1792. By April 1793, Whitney had designed and constructed the cotton gin, a machine that automated the separation of cottonseed from the short-staple cotton fiber. Advantages of Eli Whitneys Cotton Gin Eli Whitneys invention of the cotton gin revolutionized the cotton industry in the United States. Prior to his invention, farming cotton required hundreds of man-hours to separate the cottonseed from the raw cotton fibers. Simple seed-removing devices have been around for centuries, however, Eli Whitneys invention automated the seed separation process. His machine could generate up to fifty pounds of cleaned cotton daily, making cotton production profitable for the southern states. Eli Whitney Business Woes Eli Whitney failed to profit from his invention because limitations of his machine appeared and his 1794 patent for the cotton gin could not be upheld in court until 1807. Whitney could not stop others from copying and selling his cotton gin design. Eli Whitney and his business partner Phineas Miller had decided to get into the ginning business themselves. They manufactured as many cotton gins as possible and installed them throughout Georgia and the southern states. They charged farmers an unusual fee for doing the ginning for them, two-fifths of the profits paid in cotton itself. Copies of the Cotton Gin And here, all their troubles began. Farmers throughout Georgia resented having to go to Eli Whitneys cotton gins where they had to pay what they regarded as an exorbitant tax. Instead planters began making their own versions of Eli Whitneys gin and claiming they were new inventions. Phineas Miller brought costly suits against the owners of these pirated versions but because of a loophole in the wording of the 1793 patent act, they were unable to win any suits until 1800, when the law was changed. Struggling to make a profit and mired in legal battles, the partners finally agreed to license gins at a reasonable price. In 1802, South Carolina agreed to purchase Eli Whitneys patent right for $50,000 but delayed in paying it. The partners also arranged to sell the patent rights to North Carolina and Tennessee. By the time even the Georgia courts recognized the wrongs done to Eli Whitney, only one year of his patent remained. In 1808 and again in 1812 he humbly petitioned Congress for a renewal of his patent. Eli Whitney - Other inventions In 1798, Eli Whitney invented a way to manufacture muskets by machine so that the parts were interchangeable. Ironically, it was as a manufacturer of muskets that Whitney finally became rich. The cotton gin is a device for removing the seeds from cotton fiber. Simple devices for that purpose have been around for centuries, an East Indian machine called a charka was used to separate the seeds from the lint when the fiber was pulled through a set of rollers. The charka was designed to work with long-staple cotton, but American cotton is a short-staple cotton. The cottonseed in Colonial America was removed by hand, usually the work of slaves. Eli Whitneys Cotton Gin Eli Whitneys machine was the first to clean short-staple cotton. His cotton engine consisted of spiked teeth mounted on a boxed revolving cylinder which, when turned by a crank, pulled the cotton fiber through small slotted openings so as to separate the seeds from the lint a rotating brush, operated via a belt and pulleys, removed the fibrous lint from the projecting spikes. The gins later became horse-drawn and water-powered gins and cotton production increased, along with lowered costs. Cotton soon became the number one selling textile. Demand For Cotton Grows After the invention of the cotton gin, the yield of raw cotton doubled each decade after 1800. Demand was fueled by other inventions of the Industrial Revolution, such as the machines to spin and weave it and the steamboat to transport it. By mid-century America was growing three-quarters of the worlds supply of cotton, most of it shipped to England or New England where it was manufactured into cloth. During this time tobacco fell in value, rice exports at best stayed steady, and sugar began to thrive, but only in Louisiana. At mid-century the South provided three-fifths of Americas exports, most of it in cotton. Modern Cotton Gins More recently devices for removing trash, drying, moisturizing, fractioning fiber, sorting, cleaning, and baling in 218-kg (480-lb) bundles have been added to modern cotton gins. Using electric power and air-blast or suction techniques, highly automated gins can produce 14 metric tons (15 U.S. tons) of cleaned cotton an hour.

The Abbasid Caliphate

The Abbasid Caliphate The Abbasid Caliphate, which ruled most of the Muslim world from Baghdad in what is now Iraq, lasted from 750 to 1258 A.D. It was the third Islamic caliphate and overthrew the Umayyad Caliphate to take power in all but the western-most fringe of Muslim holdings at that time- Spain and Portugal, known then as the al-Andalus region. After they defeated the Ummayads, with significant Persian assistance, the Abbasids decided to de-emphasize ethnic Arabs and recreate the Muslim caliphate as a multi-ethnic entity. As part of that reorganization, in 762 they moved the capital from Damascus, in what is now Syria, northeast to Baghdad, not far from Persia in present-day Iran. Early Period of the New Caliphate Early in the Abbasid period, Islam exploded across Central Asia, although usually the elites converted and their religion trickled down gradually to ordinary people. This, however, was not conversion by the sword. Incredibly, just one year after the fall of the Umayyads, an Abbasid army was fighting against the Tang Chinese in what is now Kyrgyzstan, in the  Battle of Talas River  in 759. Although Talas River seemed like just a small skirmish, it had important consequences- it helped to set the boundary between the Buddhist and Muslim spheres in Asia and also allowed the Arab world to learn the secret of paper-making from captured Chinese artisans. The Abbasid period is considered a Golden Age for Islam. Abbasid caliphs sponsored great artists and scientists and great medical, astronomical, and other scientific texts from the classical period in Greece and Rome were translated into Arabic, saving them from being lost. While Europe languished in what was once called its Dark Ages, thinkers in the Muslim world expanded upon the theories of Euclid and Ptolemy. They invented algebra, named stars like Altair and Aldebaran and even used hypodermic needles to remove cataracts from human eyes. This was also the world that produced the stories of the Arabian Nights- the tales of Ali Baba, Sinbad the Sailor, and Aladdin came from the Abbasid era. The Fall of the Abbasid The Golden Age of the Abbasid Caliphate ended on February 10, 1258, when Genghis Khans grandson, Hulagu Khan, sacked Baghdad. The Mongols burned the great library in the Abbasid capital and killed the Caliph Al-Mustasim. Between 1261 and 1517, surviving Abbasid caliphs lived under Mamluk rule in Egypt, wielding more or less control over religious matters while having little to no political power. The last Abbasid caliph, Al-Mutawakkil III, supposedly handed over the title to the Ottoman Sultan Selim The First in 1517. Still, what was left of the destroyed libraries and scientific buildings of the capital lived on in Islamic culture- as did the pursuit of knowledge and understanding, especially concerning medicine and science. And although the Abbasid Caliphate was considered Islams greatest in history, it would certainly not be the last time a similar rule took over the Middle East.

Intergenerational Interview Essay Example | Topics and Well Written Essays - 1000 words

Intergenerational Interview - Essay Example At this state, the psychological crisis as characterized by Erickson is â€Å"integrity† versus â€Å"despair† (Shaffer & Kipp, 2009). Individuals in this stage are more associated with wisdom. For Erickson, integrity brought out two meanings. The first meaning involves consistency of values, actions, measures, methods, expectations, principles, and outcomes. It entails the ability of an individual to achieve his or her goals. The second meaning is an immanent quality of truthfulness and honesty as the major motivators of an individual’s actions. Despair is as a result of lack of hope (Zastrow & Kirst-Ashman, 2009). On reflecting on her life, Catherine feels like she has achieved enough in terms of taking care of her family. Integrity associated with this stage of life is evident in relation to Catherine when she looks at both her daughters, who are all independent, and feels successful. However, despair steps in when Catherine feels like she failed to do enough to keep her husband alive, even though there was nothing she could do, as she is very lonely. In addition, she feels like she did not make enough financial achievements and that she is going to run out of money, when in real sense she is not poor. One of the most significant developmental life events that Catherine went through was marriage. It is important to note that young adults have a need for establishing loving, intimate relationships with other individuals. The success of her marriage was highly important in her young and middle adulthood as she built a strong relationship with her husband and children. This is evident in her loneliness and her memories of her husband who died. Also, the distance of her daughters has increased her level of loneliness as they have established their families and live independently. However, the presence of her grandchildren has enabled her to establish new

What is the benefit to join the army Essay Example | Topics and Well Written Essays - 500 words

What is the benefit to join the army - Essay Example Human beings need to meet their safety needs. In effect, the assurance that the Army meets their healthcare needs inevitably makes them meet their social needs according to Maslow’s hierarchy of needs. In this regard, not only does the Army provide healthcare to its personnel but also caters for the healthcare needs of their immediate family members. Education: The ROTC program is enough evidence that there are chances in the Army to further education. In this regard, individuals should not worry that their chances of pursuing further education end after joining the Army once they completed college. In this case, members of the US military are eligible to join any education program with full tuition coverage met by the Army. Vacation and Travel: We all need time to travel and unwind once in awhile. The Army provides individuals serving thirty days leave every year. Thus, individuals will get a chance to travel and go for vacation around the world. College Loan Repayment: Some studies indicate that a majority of Americans spend a lot of time working to repay their education loans. However, the Army pays loans up to $65,000. Retirement Benefits: We all want to retire peacefully and have earnings that will sustain us through the rest of our life. The Army provides a beautiful retirement package to thank individuals for service as they integrate themselves in civilian life. This section outlines the overheads acetates that we will use to capture the attention of the program attendants in order to capture their attention. Prerequisites for admission to any ROTC program according to Go Army: It is only fair to evaluate the effectiveness of the training program by getting feedback from the audience. In this regard, the best method to use will be the method that Campbell and Stanley called the One-Shot Case Study (ac cited in Seel, 2011). In effect, we will focus on assessment and evaluation of some levels such as

Key Concepts in Management Coursework Example | Topics and Well Written Essays - 750 words

Key Concepts in Management - Coursework Example is because machines are beneficial for increasing the overall efficiency but it cannot be considered as a tool to communicate with employees (Marchington & Wilkinson, 2008, pp. 56-57). On the contrary, organization provides an environment in which employees can enhance their competencies as well as develop their skills to sustain in the workplace for longer period of time. Machines are usually subjected to decreasing rate of productivity which is not true in context of an organization. A machine can be more powerful than an organization in the initial phases but in case of sustainability it cannot be considered to be effective. Employees or living components of an organization cannot be sustained by a machine for long run. As for sustainability productivity and efficiency are not the only vital components (Torrington, Hall & Taylor, 2005, pp.110-111). An effective operation is not enough to ensure longevity as other factors such as work collaboration, high adaptability, incorporating necessary changes etc., is equally important. It can be stated that external forces play a major role in ensuring longevity of a system. Modern organizations to some extent seem to be preoccupied with the idea of maintaining status quo in the industry. However risk is a factor which cannot be eliminated completely from the system. Status quo is often considered to be the most difficult element in terms of sustainability. In the present competitive environment organizations had to strive hard in order to maintain their respective status quo in the industry. However there is often a mindset developed that organizations are performing well and has effectively maintained their status quo. This can be considered to be a prison mindset as such situation is never real in the present business environment (Jackson, Schuler &Â  Werner, 2011, pp. 101-103). In certain context this form of prison mindset develops when a firm possesses a record of outstanding performance and has a reputed image

Abortion of Fetus with Down Syndrome Essay Example | Topics and Well Written Essays - 1000 words

Abortion of Fetus with Down Syndrome - Essay Example For instance, these proponents have argued that for a fetus with Down syndrome, it is permissible to carry out abortion. Down syndrome (DS) is a health condition in which superfluous genetic material leads to delays in the mental and physical development. This paper holds that it is morally permissible to abort a fetus with Down syndrome due to various reasons. Apparently, aborting this fetus will save it future pain and suffering once born. In addition, the fetus lacks capacity for making its own decision and does not have the capacity to desire for the continuation of its existence. Moreover, the fetus lacks more developed person-like features and it lacks fully developed rights to life (Warren, 2004). Moral issues surrounding abortion. The issue of terminal conditions and abortion accrues from the idea of active euthanasia. Marquis (1989) indicates that the overall issue of killing a person who is terminally ill to save him or her from pain is sometimes not justifiable. This accrues from the premise that death is more painful that all other forms of physical pain. On the other hand, he claims that it is not generally wrong to kill the person who would die anyway. Under this premise, it is then not morally wrong to abort or terminate a fetus suffering from a terminal condition such as Down syndrome. This raises the proposition that aborting might be illegal but considering the moral responsibility concerning terminal conditions, it becomes morally justifiable. ... He adds that the parents of these children undergoes through hard financial strains while trying to provide adequate treatment to them. It is under this notion that it becomes morally permissible to abort a fetus that will probably bring about these problems once born. This premise asserts that it is not morally wrong to terminate a life that would otherwise go through pain and bring strain. He adds that there is a difference between terminally ill adult individuals and a fetus with a severe condition. This is in the sense that the adult individual can make a decision for himself or herself while the fetus does tot have the capacity to make its own decision. This therefore supports the claim that it is vital to make the decision on behalf of the fetus, thus becoming morally acceptable to abort it. On the other hand, Marquis (1989) indicates that some opponents of this issue would claim that it is not right to kill a terminally ill person due to his or her future value. Under this pre mise, the opponents would argue that this should be the same in the case of a fetus with Down syndrome. However, this assertion fails in the sense that a fetus is not fully ripe to realize its value in the future. Though there might be some innate value in the baby, there is no justification that it would add value in the future. This premise rests entirely in the public opinion but not in the reality of the issue. Another strong view in proposing the issue of aborting a 16 week- fetus with Down syndrome rests in the incompleteness of the person-like feature of the fetus. It is very evident that even though opponents of abortion argue that the fetus is a human being, it lacks unquestionable features to make it person-like. For instance, Warren (2004)

West Yorkshire Police And A Merger Research Proposal

West Yorkshire Police And A Merger - Research Proposal Example The goal being to incorporate the police together so they would ultimately be together under one unit. The proposal will investigate the issues that would arise for the public sector as a result of the mergers. In addition, the perception of the police regarding the merger will be examined. The purpose of the research on the merger is to investigate and present the action methods that will work best to formulate a decision regarding the merger and reach a conclusion as to the best and most ethical course. There are many fact ors to be taken into consideration regarding a merger.. Included in the Yorkshire Police merger are legal issues, A recent newspaper article re ported on these issues. That the government was being taken to the high court over its plans to merge police forces, while rebel Labour MPs plan to derail the scheme in the Commons. Cleveland police force is to seek a judicial review of the home secretary's plans to merge it with the Durham and Northumbria forces. Guardian Unlimited Guardian News and Media Limited 2007 It further reportd thar While this was going on a group of Labour MPs led by John Grogan intends to sabotage the merger plans when they come through the Commons in the autumn. This twin opposition is John Reid's second major headache since being appointed at the start of the month, following the debacle of the failure to deport released foreign prisoners. The idea of bigger, better resourced, forces came in the wake of criticism of the small Cambridgeshire police force's response to the Soham murders. Guardian Unlimited Guardian News and Media Limited 2007 My research concluded that a go od merger is contingent on good planning and research Law enforcement in the United Kingdom is not the responsibility of one single police service serving the general public; with the exception of various special police forces and of Northern Ireland (which has one unified force, the Police Service of Northern Ireland), police forces are arranged in geographical areas matched to the boundaries of one or more local authorities; in recent years being increasingly described as "territorial police forces". (Baskerville 2004 p 329) In turn, these forces are regulated by the laws of the appropriate country within the UK (administration of police matters is not generally affected by the Government of Wales Act 2006), i.e., Scotland, Northern Ireland, England and Wales. It is common for the territorial police forces in England and Wales to be referred to as "Home Office" police forces, after the government department which exercises control at a national level in England and Wales but this is erroneous as the description can encompass a number of miscellaneous forces subject to some kind of control by the Home Office but which are not the concern of the various Police Acts which control territorial police forces. (Bakersville 2004 p330) In 1981, James Anderton, the then Chief Constable of Greater Manchester Police called for 10 regional police forces for England and Wales, one for each of the regions which would be adopted as Government Office Regions in England, and Wales.[2] A 2004 proposal by the Police Superintendents Association for the creation of a single national police force, similar to the Garda Sochna na hireann was rejected by the Association of Chief Police Officers, and the government has thus far agreed.[3] In September 2005, in a report[4] delivered to the then Home Secretary, Charles Clarke, HM Inspectorate of Constabulary suggested that the

Abortion of Fetus with Down Syndrome Essay Example | Topics and Well Written Essays - 1000 words

Abortion of Fetus with Down Syndrome - Essay Example For instance, these proponents have argued that for a fetus with Down syndrome, it is permissible to carry out abortion. Down syndrome (DS) is a health condition in which superfluous genetic material leads to delays in the mental and physical development. This paper holds that it is morally permissible to abort a fetus with Down syndrome due to various reasons. Apparently, aborting this fetus will save it future pain and suffering once born. In addition, the fetus lacks capacity for making its own decision and does not have the capacity to desire for the continuation of its existence. Moreover, the fetus lacks more developed person-like features and it lacks fully developed rights to life (Warren, 2004). Moral issues surrounding abortion. The issue of terminal conditions and abortion accrues from the idea of active euthanasia. Marquis (1989) indicates that the overall issue of killing a person who is terminally ill to save him or her from pain is sometimes not justifiable. This accrues from the premise that death is more painful that all other forms of physical pain. On the other hand, he claims that it is not generally wrong to kill the person who would die anyway. Under this premise, it is then not morally wrong to abort or terminate a fetus suffering from a terminal condition such as Down syndrome. This raises the proposition that aborting might be illegal but considering the moral responsibility concerning terminal conditions, it becomes morally justifiable. ... He adds that the parents of these children undergoes through hard financial strains while trying to provide adequate treatment to them. It is under this notion that it becomes morally permissible to abort a fetus that will probably bring about these problems once born. This premise asserts that it is not morally wrong to terminate a life that would otherwise go through pain and bring strain. He adds that there is a difference between terminally ill adult individuals and a fetus with a severe condition. This is in the sense that the adult individual can make a decision for himself or herself while the fetus does tot have the capacity to make its own decision. This therefore supports the claim that it is vital to make the decision on behalf of the fetus, thus becoming morally acceptable to abort it. On the other hand, Marquis (1989) indicates that some opponents of this issue would claim that it is not right to kill a terminally ill person due to his or her future value. Under this pre mise, the opponents would argue that this should be the same in the case of a fetus with Down syndrome. However, this assertion fails in the sense that a fetus is not fully ripe to realize its value in the future. Though there might be some innate value in the baby, there is no justification that it would add value in the future. This premise rests entirely in the public opinion but not in the reality of the issue. Another strong view in proposing the issue of aborting a 16 week- fetus with Down syndrome rests in the incompleteness of the person-like feature of the fetus. It is very evident that even though opponents of abortion argue that the fetus is a human being, it lacks unquestionable features to make it person-like. For instance, Warren (2004)

Autonomhy in healthcare Essay Example | Topics and Well Written Essays - 5000 words

Autonomhy in healthcare - Essay Example Donchin criticized Engelhardt approach to autonomy and noted that the power and authority of physicians to act as gatekeepers selecting candidates for non-therapeutic elective procedures raised the different set of issues about limiting patient autonomy. Tauber notes that patients lose their ability to make free choices because they are never fully informed. In his perception, by employing the term of adequate understanding, the patients themselves recognize the limits of their freedom and are more concerned with the process of healing and autonomy is rather the aspiration of the curing process. Verkerk and Keller analyzed patient autonomy in the context of care perspective or feministic approach. The patient care is focused on becoming more active and committed caregivers instead of adopting the non-interference attitude and respect of patient autonomy. The ideal of autonomy in terms of independence and self-sufficiency is criticized, while the idea of interdependence is emphasized. Dworkin, on the contrary, has covered the issue of autonomy from different perspectives starting with the Kantian concept of self-governance and ending with the feministic approach to autonomy. Even though Dworkin does not use the word autonomy in his works, while discussing the idea of treating people equally he is arguing for the equal respect for the autonomy of people. For example, according to his liberal theory of equality, the political decisions should be free of any conception of the good life because the conceptions of people are different and the government will be unable to treat citizens as equals if one conception is prefer to other. Thus, autonomy is an equivalent of liberty, self-rule, integrity, individuality, and self-knowledge (6). Dworkin argues that autonomy is not the same concept as liberty. For example, if the doctor

Lord of the Flies - Darkness of a Mans Heart Essay Example for Free

Lord of the Flies Darkness of a Mans Heart Essay Originally published in 1954, William Goldings _Lord of the Flies_ has become one of the classics of contemporary literature. The novel is about a group of young boys stranded alone on an island and left to fend for themselves. In an attempt to model their previous world without the influence of adults, the order that had once existed soon decays and is replaced by the chaos that destroys their ordered and civilised cultures. Though fictional, _Lord of the Flies_ deals with deep moral questions of how humans are essentially barbaric in their most primitive state. Golding conveys his idea of the darkness of a mans heart successfully through effective use of allegory, symbolism, and his perception of a dystopian society. _Lord of the Flies_ can be viewed as a political allegory of the Cold War. Golding served in the Royal Navy and recalling later his war experiences, he remarked that man produces evil, as a bee produces honey. The Cold War was a struggle between two super power ideologies Democracy and Communism, signified by Ralph and Jack respectively. Near the close of the novel, the boys are rescued by a naval commander who came ashore in his boat whilst the boys were playing savages. The irony is that after he brings the boys home, the naval commander will return to sea and engage in a far larger game of violence called war. Thus, Golding makes a point that the island is a microcosmic representation of the conflict between democracy and communism in the war. When the boys are first deserted on the island, they behave like children, alternating between enjoying their freedom and expressing profound homesickness and fear. By the end of the novel, however, they mirror the warlike behaviour of the Home Counties, attacking, torturing and murdering one another without hesitation or regret due to the lack of government and order. Ralph weeps for the end of innocence and the darkness of a mans heart (p 248) upon reunification with the real world and realisation that evil lurks within all human beings. Symbolism plays a major role in _Lord of the Flies_. Through the rich use of symbolism, Golding reveals that humans detached from societys rules allow themselves to be dominated by the evil within them. The Lord of the Flies and the Beast are two major symbols that enforce the Goldings idea of the darkness in a mans heart. They are not really physical characters, but rather the evil in every human being.Simon is the first one on the island to realise and thus makes a proposal. What I mean is†¦maybe its only us. (p 111)Simon speaks these words in Chapter 5, during the meeting in which the boys discuss the existence of the beast. Although the other boys laugh off Simons suggestion that the beast may be only us, Simons words are central to Goldings point that innate human evil exists. Simons idea of the darkness of a mans heart is reinforced in Chapter 8, when he confronts the Lord of the Flies. There isnt anyone to help you. Only me. And Im he Beast. (p 177) These words spoken by the Lord of the Flies confirm Simons speculation that perhaps the beast is only the boys themselves and acknowledges that it exists in all human beings. This is backed up further with The Lord of the Flies adoption of the boys rather colloquial language, Im the reason why its no go? (p 177) As the story progresses, the boys begin to worship the beast and make offerings to it. The appreciation for the beast is paralleled by the amount of savagery in the group. Through the use of symbolism, Golding successfully illustrates his theme of natural savagery within humans. The concept of a dystopian society can be seen clearly through _Lord of the Flies._ Golding sees moral behaviour as something that civilisation forces upon the individual rather than being a natural element. The novel implies that the instinct of savagery is far more primal and fundamental to human beings than the instinct of civilisation.The impression of a dystopian society within _Lord of the Flies_ is enhanced by Goldings rich and dark writing style. During the early chapters of the novel, the setting was depicted as almost a utopia, Ralph stood, one hand against a grey trunk, and screwed up his eyes against the shimmering water (p 14), yet however is juxtaposed with dystopian qualities, Behind this was the darkness of the forest proper and the open space of the scar. This creates a sense of  foreshadowing which eventually leads on to the corruption and deterioration of the boys civilisation. With the absence of civilisation, the boys are left to fend on their own with minimal resources andtherefore naturally reverting cruelty, savagery, and barbarism. _Lord of the Flies_ still holds relevance to readers in this day as it addresses issues regarding the human condition and society. These issues will be as relevant today as they were hundreds of years ago and will still be relevant in the future. As for allegory, the novel represents the differences in political systems, systems that are still part of our lives today. If we scrape off the cover of civilisation, we are all beasts within.Through use of allegory, symbolism and a concept of dystopia, Golding conveys that the absence of a civilisation is able to reduce humanity to a natural state of barbarism.In conclusion, _Lord of the Flies_ thoroughly explores the darkness of a mans heart.

A Review On VBNC Bacteria

A Review On VBNC Bacteria Viable but Non-Culturable (VBNC) state is a unique survival strategy of many bacteria in environment in response to adverse environmental conditions. VBNC bacteria cant be cultured on routine microbiological media but they remain viable and retain their virulent capacity too. VBNC bacteria can be resuscitated when provided with appropriate conditions. A good number of bacteria including many human pathogens have been reported to enter VBNC state. Though, there was disputes on the existence of VBNC in past, extensive molecular studies have resolved most of them and VBNC has been accepted as distinct survival state by all. VBNC bacteria are considered as threats to public health and food safety due to their non-detectability and virulence as food and water have been reported to be contaminated with pathogens at VBNC state though conventional methods declared them as safe and clean. A number of outbreaks have also been reported where VBNC bacteria has been implicated as causative agent. Further molecular and combinatorial research in conjunction with predictive modeling are needed to elucidate the mechanisms and to identify the critical points to tackle the threat posed by VBNC bacteria with regard to public health and food safety. Key words: VBNC, Pathogen, public health, Food safety, Detection Introduction The cells that form colony in specific media are the culturable cells. Viable means metabolically or physiologically active. So the cells those are metabolically or physiologically active but cant be cultured on specific media are the viable but non-culturable cells (VBNC) (Bogosian Bourneuf, 2001). Most microorganisms growing in nature have yet to be cultured in the laboratory. In fact less than 1% of the microorganisms in natural water and soil samples are cultured in viable count procedures (Barcian Arana, 2009). In 1982, Prof. Rita Colwell and co-workers introduced the term Viable But Non-Culturable Bacterial Cells (VBNC) to distinguish particular cells that could not form colonies on solid media but obtained metabolic activity and the ability to elongate after the administration of nutrients (Xu et al., 1982). According to Oliver (1995), VBNC can be defined as a metabolically active bacterial cell that crossed a threshold in this way, for known or unknown reasons and become unable to multiply in or on a medium normally supporting its growth. Most of the bacteria that enter VBNC state are gram negative species belonging to the gamma subclass of the Proteobacteria branch, except for Rhizobium, Agrobacterium and Helicobacter-Campylobacter species (Oliver, 2000). History Debra Bashford and colleagues announced that they had recovered Vibrio cholerae from streams and drainage ditches, including sites with negligible chance of sewage contamination. Around the same lime, Rita Colwell was also finding Vibrio cholerae in Maryland. She and her coworkers showed that both this bacterium and E. coli, incubated in artificial sea water remained viable but lost the capacity to form colonies on culture media (Colwell Grimes, 2000). Soon Salmonella enteritidis, Shigella sonnie and Legionella pneumophila joined the list of organisms known to be capable of entering a state in which they failed to show up on nutrient agar yet took up substrates and signaled in other ways that they were certainly not dead. The use of laboratory media to recover and enumerate bacteria and lo link them with or absolve them from pathological and other activities became obsolete by the new discoveries and a term VBNC (viable but non-culturable) came (McDougald et al., 1998). VBNC Microorganisms that do not grow in culture methods, but which are still metabolically active and capable of causing infections in animals and plants are said to be in VBNC state. Traditional laboratory culture conditions methods cannot meet the requirements of VBNC organisms to resume growth (Yamamoto, 2000). Semi-starved bacteria usually resume growth immediately when appropriate nutrients conditions are provided. Viable but non-culturable cells will not resume growth even when nutrients are provided (NystrÃÆ'-m, 2001). VBNC cells exhibit active metabolism in the form of respiration or fermentation, incorporate radioactive substrates, and have active protein synthesis but cannot be cultured or grown on conventional laboratory media. They have been detected by observing discrepancies between plate count enumeration of bacterial population and direct staining and microscopic counts (Sachidanandham Gin, 2009). These cells may be of particular problems in the environment if they are pathogens, for example, viable but non-culturable cells of Vibrio cholerae, Enteropathogenic E. coli, Legionella pneumophila and various other bacteria have been shown to regain culturability after they have entered the intestinal tracts of animals (Colwell et al., 1996). The VBNC state is defined as a state of dormancy triggered by environmental harsh conditions, such as nutrient starvation (Cook Bolster, 2007), temperature (Besnard et al., 2002), osmotic stress (Asakura et al., 2008), oxygen availability (Kana et al., 2008), several food preservatives (Quirà ³s et al., 2009), heavy metals (Ghezzi Steck, 1999), exposure to white light (Gourmelon et al., 1994) and decontaminating processes, as pasteurization of milk (Gunasekera et al., 2002) and chlorination of wastewater (Oliver, 2005). VBNC state is believed to be a unique survival strategy of bacteria in response to environmental stresses (Oliver, 2010). It is also considered as an important reservoir of many human pathogens in the environment (Lleo et al., 2007). VBNC state has been a matter of dispute for ling since its inception, due to the difficulty of differentiation of VBNC cells dormant cells through resuscitation phenotypic studies, recent molecular studies, data of which supported the existence of VBNC state, the dispute has mostly been put to rest (Barer and Harwood, 1999). VBNC Pathogens Following list includes but not limited to pathogenic bacteria that can enter VBNC state (Oliver, 2010)- Aeromonas hydrophila, Agrobacterium tumefaciens, Burkholderia cepacia, Campylobacter jejuni, Enterobacter aerogenes, Enterobacter cloacae, Enterococcus faecalis, Escherichia coli (including EHEC), Helicobacter pylori, Klebsiella pneumoniae, Legionella pneumophila, Listeria monocytogenes, Mycobacterium tuberculosis, Pseudomonas aeruginosa, Salmonella typhi, Salmonella typhimurium, Shigella dysenteriae, Shigella flexneri, Shigella sonnei, Streptococcus faecalis, Vibrio alginolyticus, Vibrio cholerae, Vibrio harveyi, Vibrio parahaemolyticus, Vibrio vulnificus (types 1 and 2) Characteristics of Bacteria in VBNC state 1. Maintain apparent cell integrity; 2. possession of some form of measurable cellular activity (Lai et al., 2009); 3. possess apparent capacity to regain culturability (Anuchin et al., 2009); 4. respond to external stimulus by specific gene expression (kell et al., 1998); 5. low metabolic activity (oliver, 2005); 6. Exhibit dwarfing (Costa et al., 1999); 7. reduced nutrient transport; 8. High ATP level and high membrane potential (Signoretto et al., 2000); 9. extensive modifications in cytoplasmic membrane fatty acid compositions (Day Oliver, 2004); 10. Changes in cell wall peptidoglycan such as increasing cross linking, increasing muropeptides bearing covalently bound lipoprotein shortening of average length of glycan strands (signoretto et al., 2002); 11. Higher autolytic capability than exponentially growing cells; 12. Plasmids are retained; 13. changed antibiotic sensitivity as metabolic activity is lower, most bacteria at VBNC state demonstrate high antibiotic resistance (Ol iver, 2010); 14. Changes in outer-membrane protein profile (Muela et al., 2008); 15. Continuous gene expression (Maalej et al., 2004) etc. Conditions stimulating VBNC state In the environment, bacterial cells can enter VBNC state may be due to- 1. Lack of nutrients; 2. Lack of temperatures; 3. High pressure; 4. Sharp changes in pH or salinity (Cunningham et al., 2009); 5.damage to or lack of an essential cellular component; 6. DNA damage; 7. activation of lysogenic phages or suicide genes such as sok/hak or autolysins (Aizenman et al., 1996); 8. Nutrient starvation; 9. incubation outside the normal temperature range of growth; 10. elevated or lower osmotic concentrations; 11. oxygen concentrations (Mascher et al., 2000); 12. food preservatives; 13. Heavy metals (Del Campo et al., 2009); 14. exposure to white light; 15. pasteurization of milk (Gunasekara et al., 2002); 16. chlorination of wastewater (Oliver, 2005) etc. Public health significance of VBNC Though virulence of bacteria in VBNC state is still not very clear, many believed that pathogens in VBNC state are unable to induce infection/disease but still retain their virulent properties has potential to cause disease infection following resuscitation and resume of active metabolic state, which occurs when they pass through host animal (Baffone et al., 2003). The VBNC state appears to be the common to many bacteria especially those which have aquatic habitats, and may represent a mechanism to survive adverse environmental factors as temperature, salinity etc. or have a means of inducing cross protection against other adverse factors (Du et al., 2007). Among these bacteria entering this state are many significant human pathogens and indicator bacteria of these pathogens; such cells may represent a public health hazard and may be a factor in human health and/or disease (Rivers Steck, 2001). Even today, it is still not possible to cultivate most bacterial species directly from the environmental samples or after exposure of previously culturable cells to environmental conditions unfavorable for growth and multiplication in vitro. The passage of VBNC through an appropriate animal host will induce return of culturability. Even these VBNC bacteria retain their pathogenicity and may trigger life in vivo and thus cause severe disease (Sardessai, 2005). Under normal condition it is not possible to culture or detect VBNC. Many diagnostic laboratory set up does not have sufficient molecular facilities to detect VBNC. In case of food and water quality control test, such VBNC may not be detected. Even some indicator of some pathogenic bacteria undergoes VBNC state and may remain undetected (Signoretto et al., 2004). Upon consuming such food or after drinking such water, one may be infected by those VBNC that can trigger life as well as pathogenicity (Adams et al., 2003). Thus, environmental and clinical samples no longer can be considered free from pathogens if culturing yields negative results. For the general public, the presence of VBNC in water and food may be related to low-grade infections or so called aseptic infection. For example, Vibrio cholerae O1 in the surface water remain as non-culturable state. These water sources are used for domestic purpose regularly and posed a risk of infection (Edwards, 2000). When conditions are not favorable for growth then it transforms to the non-culturable state in association with crustacean copepods. Persistence of Vibrio cholerae in water in the VBNC state is an important public health factor, since detection will not be successful if only conventional cultural methods are used (Barer et al., 1993). Similarly, Shigella can undergo VBNC state in water but become a threat when enter in human body. Thus it is important to recognize that non-culturable bacteria are capable of producing diseases. The first evidence of pathogenicity of non-culturable cells was the demonstration of fluid accumulation in rabbit ileal loop assay (RICA) by VBNC Vibrio cholerae O1, followed by human volunteer experiments (Amel et al., 2008). VBNC E. coli non-culturable cells were re-isolated after passage through rabbit ileal loops 4 days post inoculation and chick embryos died when injected with non-culturable cells of Legionella pneumophila, led to the conclusion that VBNC pathogens remain potentially pathogenic. So, VBNC has a huge significance in public health care (Cappelier et al., 2007). Previous studies indicated that a good number of pathogenic bacteria can survive food water treatment processes persist as well as retain virulence in processed food, pasteurized milk, potable water environment (Colwell et al., 2000). Many evidences suggested that recurrent urinary tract infections in many individuals are caused by uropathogenic E. coli cells which remain in VBNC state (Anderson et al., 2004) thus resistant to antibiotic treatment cause reinfection when resuscitate back to active metabolic state (Steck, 2001; Mulvey et al., 2001). Studies also showed that uropathogenic E. coli retain enteropathogenicity at VBNC state through continued production of enterotoxin (Pommepuy et al., 1996). Nilsson et al. (2002) showed that VBNC Helicobacter pylori cells can express virulence factors such as cagA, vacA and vreA. All these above evidence proved that many deathly pathogenic strains not only enter but also persist survive in VBNC state in environment most of them remain infectious as well. VBNC state of foodborne bacteria- a challenge in food safety Many evidences suggested presence of VBNC bacteria in food (Ordax et al., 2009). For example, in stored wine, acetic acid and lactic acid bacteria entered VBNC state as consequence of lack of oxygen and presence of sulphites, respectfully (Millet and Lonvaud-Funel, 2000). Food and its surrounding environment is a complex system, in which physic-chemical characterisitcs (pH, aw, chemical composition) and environmental factors (storage temperature and time, decontamination treatments, packaging under modified atmosphere) act simultaneously on contaminating bacteria (Sun et al., 2008). For example, it has been demonstrated that refrigerated pasteurized grapefruit juice induced VBNC state in E. coli O157:H7 and S. typhimurium within 24 hours of incubation (Nicolo et al., 2011). Again, Gunasekera et al. (2002) reported that in pasteurized milk which have undergone thermal treatment, contaminating bacteria such as E. coli and Pseudomonas putida enter into VBNC state but retained transcription and translation machineries. Several foodborne outbreaks has been reported in Japan, where pathogen such as Salmonella enterica subsp. enterica (Asakura et al., 2002) and E. coli O157 (Makino et al., 2000) in food in VBNC state were responsible for the outbreak. Therefore, the role of food and treatment for food preservation in induction of VBNC state has to be elucidated. Predicitve models offered by biomathematics and bioinformatics would be very helpful tools, in order to evaluate the possibility that, under certain conditions, pathogen bacteria contaminating a tipology of food may enter the VBNC state (Fakruddin et al., 2012). Methods of detection of VBNC bacteria 1. Bright Field Microscopy with Nalidixic acid For detection of Bright-field or light microscopic is usually used. Cell division inhibitor such as nalidixic acid (20-40 mg/L) is used to stop cell division. After such treatment the viable cells, which actively growing, will be appeared as lengthen and the non-viable/ metabolically inactive cell will remain as it is. The cells are then observed under microscope. Viable cells will be seen as elongated whereas VBNC/ dormant cells will be seen as oval and large. 2. Fluorescent Microscopy Various fluorescent staining procedures are used in combination with other procedure to determine VBNC organisms. Frequently used stains are Acridine orange, 4,6- Diamino-2-phenyl indole (DAPI), Fluorescein isothiocyanante (FITC), Indophenyl-nitrophenyl-phenyl tetrazolium chloride (INT), 5-cyano-2,3-ditolyl tetrazolium chloride (CTC) etc (Villarino et al., 2000). Table: Fluorescent dyes used for detection of VBNC bacteria Dye Mechanism Reaction Acridine orange Acridine orange stain cells depending on the ratio of DNA to protein in the cells Actively reproducing cells appear green but slow-grower or non-reproducing cells at time of staining appear orange Di-amino-phenyl-indole (DAPI) Living cells look green under fluorescent microscope Indophenyl-nitrophenyl-phenyl tetrazolium chloride (INT) INT deposit red dye in cells that have active dehydrogenase and thus determine which of the observed cells are metabolically active INT react with dehydrogenase enzyme to produce formazone and red color, thus living cells appear red. Nalidixic acid (NA) Lengthen metabolically active cells, VBNC cells remain as it is Cells that are dividing appear to be longer in size than VBNC Fluorescein isothiocyanante (FITC) Enzyme activity in living cell FITC stain living cells violet or blue In recent years, a new differential staining assay, the BacLight ® Live/Dead assay, has been developed. The assay allows to simultaneously count total and viable (metabolically active) cells, by using two nucleic acid stains, that is green-fluorescent SYTO ® 9 stain and red-fluorescent propidium iodide stain. SYTO ® 9 propidium iodide has significant difference in their cell membrane penetration capability. SYTO ® 9 stains both live and dead bacteria whereas propidium iodide penetrates only bacteria having damaged membranes. When used together, propidium iodide reduces SYTO ® 9 fluorescence in staining dead bacteria with damaged membranes. As a result, viable bacteria with intact membranes fluoresce green while dead bacteria with damaged membranes fluoresce red (Rowan, 2011). 3. Gene probe / oligonucleotide probe / hybridization: Molecular analysis can also be used to study non-culturable microorganisms in nature. Oligonucleotide probes of l8-20 nucleotides are proving most useful because they hybridize rapidly to specific DNA sequences of target organisms. These gene probes can reveal closely related organisms or organisms with similar functional capabilities. Especially useful for the analyses of rRNA that demonstrate the presence of diverse microbial populations whose phylogenetic relationships can be ascertained by comparison with rRNA sequences from previously described microorganisms (Josephson et al., 1993). Blotting: There are different types of blotting such as colony blot, slot blot, dot blot and southern blot. The principle of blotting is the use of radio- or non-radioactive or fluorescence labeled probe (DNA/RNA/Antibody) to detect VBNC cells directly from the environmental samples. Fluorescent in situ Hybridization (FISH): In situ hybridization is an alternative format for hybridization probes in which fluorescence labeled DNA or RNA probes are hybridized with target nucleic acids in whole, permeabilized cells. The application of this method to the detection of single microbial cells by using rRNA-targeted probes in combination with epifluorescent microscopy has been developed. This is done through selective targeting of regions of rRNA, which consist of conserved and variable nucleotide regions. By choosing the appropriate rRNA probe sequence, FISH can be used to detect all bacterial cells (a universal probe) or a single population of cells (a strain specific probe) of VBNC. It has lower sensitivity and cannot distinguish live and dead cells. 4. Molecular techniques: Hybridization probes and DNA/RNA amplification: Hybridization probes are nucleic acids (DNA/RNA) which have been (a) chemically or radioactively labeled and are used to detect complementary target DNA/RNA. Hybridization assay DNA/ RNA probes form a stable double stranded structure with target nucleic acid via H-bonding between complementary bases. Amplification of targets: DNA Based methods: Specific amplification of DNA targets in bulk DNA extracts from environmental and clinical samples permits detection of specific organisms or groups of related organisms without the need to cultivate them. DNA recovery procedures do not discriminate between culturable and non-culturable forms of the target organisms- all cells with intact amplification targets will be detected. Confocal laser microscopy in combination with fluorescence-based hybridization assays, also provide a more sensitive method for detecting and identifying VBNC organisms. RNA based methods: Due to the failure of distinguishing between dead or live cells by DNA-based methods, the mRNA level may be a valuable estimate of gene expression and/or cell viability under different conditions (Lleo et al., 2000). RT-PCR: RT-PCR (Reverse Transcriptase PCR) can distinguish between Live and dead cell. This is possible because this is an mRNA based method and mRNA is short lived (half-life less than 1 minute), mRNA is only present in metabolically active cells, not found in nature after the cell death. By this method we can study community relationship and can also detect non-culturable but active or live cells. DNase enzyme is used during the isolation of RNA from environmental samples. Reverse transcriptase and random primers are added to the reaction mixture and the RNA in the sample (both RNA and rRNA) is transcribed into DNA. PCR is then use to amplify the specific sequence of interest (Pai et al., 2000). Is the concept of VBNC is a misnomer? By extending the concept of bacterial self-suicide scientists tried to explain what happens when cells are exposed to chemical and physical injury (Forsman et al., 2000). Thus VBNC organisms came alongside with those, which do not grow in ordinary media but which do grow when offered selective or enrichment media. They said, Such cells are not un-culturable they wrote We are simply failing to provide appropriate conditions to support culture (Sinton, 2006). The reasons, which made the term, VBNC a misnomer are as follow: 1. VBNC bacteria semi-starved bacteria very often mimic each other. Semi-starved bacteria resume growth if provided with appropriate nutrients conditions. But viable but non-culturable bacteria do not resume growth even though nutrients are provided. VBNC cells become too starved to grow on nutrient rich medium directly. This phenomenon resembles a widely accepted condition termed substrate accelerated death (Heim et al., 2002). These starved VBNC cells require an adjustment period to allow phenotypic adaptation back to normal growth state (Epstein, 2009). Sudden shift to nutrient enriched media imbalances metabolic networks of the cells resulting formation of DNA damage agents such as super-oxide free radicals causing cell death (Barer Harwood, 1997). 2. There is yet no complete and perfect media to isolate arid culture all the organisms from environment. 3. Cells are usually-injured or stressed or starved condition in natural environment. So complete system has been devised to enrich or resuscitated the VBNC cells. Culture condition that can be applied in laboratory is not sufficient to recover all microorganisms i.e. yet it is not possible to provide or stimulate exact environmental conditions in the laboratory. Conclusion From the above discussions, it is evident that a number of non-spore forming human pathogenic bacteria enter VBNC state with maintained cellular structure biology persistent gene expression but remain non-culturable by traditional cultural techniques. Thet can survive revert to culturable conditions when provided with appropriate conditions. It is also evident that VBNC bacteria pose significant threat to both public health and food safety. Further research is needed to elucidate the mechanism to combat the threat of VBNC in future.